antibody

Interleukin 17 (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocyte-associated antigen 8)

I8439-04K

100 ug

polyclonal

domestic goat

nonconjugated

Interleukin 17 (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocyte-associated antigen 8)

goat

Pab

Antibodies

100 ug

IgG

Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. Interleukin-17 (IL-17) and its cognate receptor, IL- 17R, are examples of cytokine receptor pairs. IL-17 protein exhibits a striking degree of homology with the HSV13 protein which mimics its function. Applications: Suitable for use in Neutralization, Western Blot, Immunocytochemistry, Flow Cytometry, Direct ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Neutralization: The ND50 is 0.02-0.12ug/ml in the presence of 15ng/ml of rhIL-17, using theNIIH/3T3 mouse embryonic fiboblasts Immunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood mononuclear cells treated with PHA Western Blot: 1ug/ml using human primary naive CD4+ T cells and human primary differentiated Th17 cells Flow Cytometry: 2.5ug to label 1x10e6 cells using human peripheral blood mononuclear cells treated with PHA and Ca2+ ionomycin, fixed with paraformaldehyde and permeabilized with saponin Immunoprecipitation: 3ug/100ug cell lysate using human primary differentiated Th17 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Recombinant protein corresponding to aa20-155 from human IL-17 expressed in E. coli (Q16552)

Recognizes human IL-17. Species Crossreactivity: rcIL-17; ~60%, rhIL-17F and rmIL-17; ~10%, rhIL-17B, rhIL;-17C, rhIL-17D, rhIL-17E; <1%

Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

~0.2mg/ml (after reconstitution)

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug Immunogen: Recombinant protein corresponding to aa20-155 from human IL-17 expressed in E. coli (Q16552) Specificity: Recognizes human IL-17. Species Crossreactivity: rcIL-17; ~60%, rhIL-17F and rmIL-17; ~10%, rhIL-17B, rhIL;-17C, rhIL-17D, rhIL-17E; <1% Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IC IP N WB

-20°C