This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
catalog :
I8432-02J
quantity :
500 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CC320

The same clone is also sold as:
reactivity :
bovine
product information
Catalog Number :
I8432-02J
Product wo Prefix :
Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
500 ug
Clone # USB :
CC320
Isotype :
IgG1
Desc1 :
Clone CC320 has been shown to neutralize the activity of bovine IL-10 as measured by the inhibition of the inhibitory activity of IL-10 on IFN gamma synthesis (Buza et al. 2004). Applications: Suitable for use in Flow Cytometry, ELISA and Functional Assays. Other applications not tested. Recommended Dilution: ELISA: 5-10ug/ml Flow Cytometry: membrane permeabilization is required for this application Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Bo Eq Gt Sh
Immunogen :
Plasmid cDNA encoding bovine IL10.
Specificity :
Recognizes bovine Interleukin 10. Species Crossreactivity: equine, sheep and goat
Purity :
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form :
Supplied as a liquid in PBS. No preservative added.
Concentration :
~1mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1 Clone No: CC320 Host: mouse Source: bovine Concentration: ~1mg/ml Form: Supplied as a liquid in PBS. No preservative added. Purity: Purified by Protein A affinity chromatography from tissue culture supernatant. Immunogen: Plasmid cDNA encoding bovine IL10. Specificity: Recognizes bovine Interleukin 10. Species Crossreactivity: equine, sheep and goat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC
Storage Temperature :
-20°C
Reference :
1. Kwong, L. S. et al. (2002) Development of an ELISA for bovine IL10.Vet. Immunol. Immunopathol. 85: 213 223.2. Buza, J.J. et al. (2004) Neutralization of interleukin10significantly enhances gamma interferon expression inperipheral blood by stimulation with Johnin purified protein derivative and by infection with Mycobacterium aviumsubsp.paratuberculosis in experimentally infected cattle with paratuberculosis. Infect Immun. 72(4):24258.
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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