Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF) | US Biological I8432-02H product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

catalog :

I8432-02H

quantity :

500 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CC318

The same clone is also sold as:

OriGene: AM05874PU-N
Invitrogen: MA5-28462
Bio-Rad: MCA2110

reactivity :

bovine

product information

Catalog Number :

I8432-02H

Product wo Prefix :

Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

500 ug

Clone # USB :

CC318

Isotype :

IgG2b

Desc1 :

Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 5-10ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Eq Sh

Immunogen :

Plasmid cDNA encoding bovine IL10.

Specificity :

Recognizes bovine Interleukin 10. Species Crossreactivity: equine and sheep

Purity :

Purified by Protein A affinity chromatography from tissue culture supernatant.

Form :

Supplied as a liquid in PBS, 0.09% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: CC318 Host: mouse Source: bovine Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 0.09% sodium azide. Purity: Purified by Protein A affinity chromatography from tissue culture supernatant. Immunogen: Plasmid cDNA encoding bovine IL10. Specificity: Recognizes bovine Interleukin 10. Species Crossreactivity: equine and sheep Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10. Vet Immunol Immunopathol. 85 (3-4): 213-23. 2. Scandurra, G.M. et al. (2009) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages. Infect Immun. 78: 1383-9. 3. Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium. J Leukoc Biol. 83 (1): 48-55. 4. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horse is associated with a change in IL-4 producing T cells. Int Arch Allergy Immunol. 144:325-37. 5. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis. J Dairy Sci. 93: 2458-70. 6. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis. Funct Integr Genomics. 10:21-38. 7. Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea. Clin Experiment Ophthalmol. 38: 405-13. 8. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification. Vet Res. 42:19. 9. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection. Clin Diagn Lab Immunol. 11: 463-72. 10. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses. Vet Res. 41: 14. 11. den Hartog, G. et al. (2011) Modulation of human immune responses by bovine interleukin-10. PLoSone 6: e18188 12. Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro. Vet Res. 44: 59. 13. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848. PLoS One. 5: e13705. 14. Jones, G.J. et al. (2010) Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle. Clin Vaccine Immunol. 17: 1946-51.