antibody

Interleukin 2 (IL-2)

I7663-27J

500 ug

monoclonal

rat

nonconjugated

MQ1-17H12

The same clone is also sold as:

• BioLegend: 500311, 500353, 500319, 500333, 500339, 500354, 500357, 500347, 500328, 500326, 500346, 500305, 500349, 500338, 500351, 500334, 500352, 500310, 500362, 500341, 500331, 500360, 500344, 500348, 500315, 500321, 500322, 500355, 500314, 500356, 500324, 500327, 500320, 500337, 500302, 500345, 500359, 500343, 500361, 500325, 500306, 500301, 500309, 500304, 500350, 500342, 500307, 500332
• BD Biosciences: 554563, 561054, 560709, 554562, 554567, 564165, 554566, 551383, 560902, 560707, 560708, 559334
• Invitrogen: 58-7029-42, 404-7029-42, 14-7029-81, 363-7029-42, 366-7029-42, 366-7029-41, 404-7029-41, 11-7029-81, 12-7029-81, 414-7029-41, 367-7029-42, 25-7029-41, 14-7029-85, 363-7029-41, 17-7029-41, 25-7029-42, 58-7029-41, 367-7029-41, 11-7029-82, 61-7029-42, 46-7029-42, 12-7029-41, 414-7029-42, 48-7029-42, 17-7029-82, 12-7029-42, 11-7029-42, 12-7029-82
• Bio-Rad: MCA1553, MCA1553A647, MCA1553A488, MCA1553PE, MCA1553F
• OriGene: SM2088F, SM2088R, SM2088P

Interleukin 2 (IL-2)

rat

Mab

Antibodies

500 ug

MQ1-17H12

IgG2a

IL-2 is a 153 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation. Interleukin-2, clone 3H2514 has been sucessfully used for the detection and measurement of native and recombinant human IL-2 using flow cytometry and ELISA where it acts as the capture reagent in a very sensitive assay for IL-2 determination in human samples. IL-2 is used extensively for the treatment of patients with metastatic melanoma (Atkins et al. 1999) and renal cancer with reproducible benificial effects (Rosenberg 2014). Monitoring of IL-2 levels in such patients is required for optimization of treatment regimens (Lotze et al. 1986). This antibody is also useful for evaluating levels of IL-2 in patients with autoimmune conditions including lupus erythematosus and rheumatoid arthritis where there may be an impaired ability to produce IL-2 (Talal et al. 1983). Applications: Suitable for use in ELISA, Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: use 10ul label 1x10e6 cells in 100ul. Membrane permeabilization is required. Optimal working dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk

Recognizes human interleukin-2 (IL-2). Species Crossreactivity: Cynomolgus monkey.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

~1mg/ml

Product Type: Mab Isotype: IgG2a Clone No: MQ1-17H12 Host: rat Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein G affinity chromatography from tissue culture supernatant. Specificity: Recognizes human interleukin-2 (IL-2). Species Crossreactivity: Cynomolgus monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC

-20°C

1. Garbe, Y. et al. (2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses. BMC Cancer. 11: 410. 2. Moncrieffe, H. et al. (2010) High expression of the ectonucleotidase CD39 on T cells from the inflamed site identifies two distinct populations, one regulatory and one memory T cell population. J Immunol. 185 (1): 134-43.