This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Interleukin 1 beta (IL-1b) (Biotin)
catalog :
I7663-07A
quantity :
50 ug
clonality :
polyclonal
host :
domestic goat
conjugate :
biotin
reactivity :
dogs
product information
Catalog Number :
I7663-07A
Product wo Prefix :
Interleukin 1 beta (IL-1b) (Biotin)
Host :
goat
Product Type :
Pab
Conjugate :
Biotin
Category :
Antibodies
Size1 :
50 ug
Isotype :
IgG
Desc1 :
Applications:
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant canine IL-1beta is ~0.5ng/lane under non-reducing and reducing conditions. Shows ~40% cross-reactivity with recombinant feline IL-1beta, 10% cross-reactivity with recombinant human IL-beta, rrmIL-1beta, recombinant equine IL-1 beta, recombinant porcine IL-1beta, and 5% cross-reactivity with rmIL-1beta, recombinant rat IL-1beta, and rcrIL-1beta.
Flow Cytometry: Dilute to 50ug/ml. Use 10ul of dilution to 1-2.5x10e5 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS, 0.1% BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Ca
Immunogen :
E. coli-derived recombinant canine IL-1beta, Asp114-Ser265.
Specificity :
Recognizes canine Interleukin 1 beta (IL-1b).
Purity :
Purified by affinity chromatography.
Form :
Supplied as a lyophilized powder from PBS and BSA. Labeled with Biotin. Reconstitute with sterile TBS (20mM Trizma vase, 150mM NaCl), 0.1% BSA.
Concentration :
Not determined
Desc2 :
Product Type: Pab
Isotype: IgG
Host: goat
Source: canine
Concentration: Not determined.
Form: Supplied as a lyophilized powder from PBS and BSA. Labeled with Biotin. Reconstitute with sterile 40-50% glycerol, TBS (20mM Trizma vase, 150mM NaCl), 0.1% BSA.
Purity: Purified by affinity chromatography.
Immunogen: E. coli-derived recombinant canine IL-1beta.
Specificity: Recognizes canine Interleukin 1 beta (IL-1b).
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC WB
Storage Temperature :
-20°C
company information
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
service@usbio.net
https://www.usbio.net800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.
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