antibody

Interferon Regulatory Factor 8 (ICSBP1, interferon consensus sequence binding protein 1, IRF8, IRF-8, H-ICSBP, interferon regulatory factor 8)

I7662-93A

100 ug

polyclonal

domestic goat

nonconjugated

Interferon Regulatory Factor 8 (ICSBP1, interferon consensus sequence binding protein 1, IRF8, IRF-8, H-ICSBP, interferon regulatory factor 8)

goat

Pab

Antibodies

100 ug

IgG

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Western blot: Approx 45-50kD band seen in K562 lysate (Predicted MW of 47kD according to NP_002154). Recommended for use at 0.5-2ug/ml. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See I7662-93A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

Hu

Synthetic peptide with sequence C-QRSFFRENQQITV, from the C-Terminus of the protein sequence according to NP_002154.1. Species sequence homology: mouse, rat and bovine

Recognizes human IRF8.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

~0.5mg/ml

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide with sequence C-QRSFFRENQQITV, from the C-Terminus of the protein sequence according to NP_002154.1. Species sequence homology: mouse, rat and bovine Specificity: Recognizes human IRF8. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Weisz A, Marx P, Sharf R, Appella E, Driggers PH, Ozato K, Levi BZ. , Human interferon consensus sequence binding protein is a negative regulator of enhancer elements common to interferon-inducible genes., J Biol Chem. 1992 Dec 15; 267(35):25589-96.

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