Interferon Regulatory Factor 4 (IRF4, Lymphocyte-specific interferon regulatory factor, NF-EM5, Multiple myeloma oncogene 1) | US Biological I7662-71J product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Interferon Regulatory Factor 4 (IRF4, Lymphocyte-specific interferon regulatory factor, NF-EM5, Multiple myeloma oncogene 1)

catalog :

I7662-71J

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

10E34(D43H10)

reactivity :

human

product information

Catalog Number :

I7662-71J

Product wo Prefix :

Interferon Regulatory Factor 4 (IRF4, Lymphocyte-specific interferon regulatory factor, NF-EM5, Multiple myeloma oncogene 1)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

10E34(D43H10)

Isotype :

IgG

Desc1 :

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-induc-ible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3gamma/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7 and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA binding domains. IRF family members regulate transcription through interac-tions with proteins that share similar DNA binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2). IRF-4 was independently cloned by three groups and demonstrated to have roles in different contexts of lymphoid regulation (3-5). First, IRF-4 (Pip) was found to associate with PU.1, a hematopoietic specific member of the ETS fam-ily, and to regulate the expression of B-cell specific genes (3). Second, it was characterized as a lymphoid-specific member of the IRF family (LSIRF) able to bind to ISRE (4). Third, it was identified in activated T cells as a factor that binds to the promoter of the interleukin-5 gene (ICSAT), and shown to repress gene activation induced by IFN (5). IRF-4 is expressed in all stages of B cell development and in mature T cells, and is inducible in primary lymphocytes by antigen mimetic stimuli such as concavalin A, CD3 cross-linking, anti-IgM and PMA treatment (4,5). Mice deficient in IRF-4 show normal distribution of B and T lymphocytes at 4 to 5 weeks, but later develop progressive generalized lymphadenopathy, suggesting a role for IRF-4 in the function and homeostasis of mature B- and T-lymphocytes (6). Applications: Suitable for use in ChIP, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 ChIP: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Rt

Immunogen :

Synthetic peptide corresponding to residues around Asp175 of human IRF-4.

Specificity :

Recognizes endogenous levels of total human IRF-4 protein. Both alternatively spliced isoforms are detected. Does not cross-react with other family members at physiological levels. Species crossreactivity: rat.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 10E34(D43H10) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues around Asp175 of human IRF-4. Specificity: Recognizes endogenous levels of total human IRF-4 protein. Both alternatively spliced isoforms are detected. Does not cross-react with other family members at physiological levels. Species crossreactivity: rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

ChIP IP WB

Storage Temperature :

-20°C

Reference :

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55, 2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58, 3. Eisenbeis, C.F. et al. (1995) Genes Dev 9, 1377-87, 4. Matsuyama, T. et al. (1995) Nucleic Acids Res 23, 2127-36, 5. Yamagata, T. et al. (1996) Mol Cell Biol 16, 1283-94, 6. Mittrücker, H.W. et al. (1997) Science 275, 540-3.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/24/I7662-71J_1.jpg