Integrin, beta5 | US Biological I7661-43D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Integrin, beta5

catalog :

I7661-43D

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

I7661-43D

Product wo Prefix :

Integrin, beta5

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Integrins are a/b heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 a and 8 b subunits that form 24 known integrins having distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2). The aVb5 integrin is expressed in various tissues and cell types, including endothelia, epithelia and fibroblasts (4). It plays a role in matrix adhesion to VN, FN, SPARC and bone sialoprotein (5) and functions in the invasion of gliomas and metastatic carcinoma cells (6,7). aVb5 integrin plays a major role in growth-factor-induced tumor angiogenesis, where cooperative signaling by the aVb5 integrin and growth factors regulates endothelial cell proliferation and survival (8). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to residues surrounding Lys789 of human integrin b5 protein.

Specificity :

Recognizes endogenous levels of total human integrin b5 protein at ~90kD.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Lys789 of human integrin b5 protein. Specificity: Recognizes endogenous levels of total human integrin b5 protein at ~90kD. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF IP WB

Storage Temperature :

-20°C

Reference :

1. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100. 2. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571. 3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298. 4. Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111. 5. Plow, E.F. et al. (2000) J. Biol. Chem. 275, 21785-21788. 6. Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166. 7. Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072. 8. Alghisi, G.C. and Ruegg, C. (2006) Endothelium 13, 113-135.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/10/I7661-43D_1.jpg