antibody

Insulin-Like Growth Factor I Receptor, beta (IGF1Rb)

I7661-21N

100 ul

polyclonal

domestic rabbit

nonconjugated

Insulin-Like Growth Factor I Receptor, beta (IGF1Rb)

rabbit

Pab

Antibodies

100 ul

IgG

Type I insulin-like growth factor receptor (IGF1R), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant similarity with IGF-I receptors in both structure and function. There is also an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151). Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4ºC with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:2400. Use EDTA as unmasking buffer. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to the C-terminal residues of human IGF1Rb.

Recognizes endogenous levels of human Insulin-Like Growth Factor I Receptor beta at ~95kD. Does not crossreact with insulin receptor. Species Crossreactivity: mouse, rat and monkey.

Purified by Protein A and peptide affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the C-terminal residues of human IGF1Rb. Specificity: Recognizes endogenous levels of human Insulin-Like Growth Factor I Receptor beta at ~95kD. Does not crossreact with insulin receptor. Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IHC IP WB

-20°C

1. Adams, T.E., et al., Cell. Mol. Life Sci. 57: 1050–1093 (2000). 2. Baserga, R., et al., Oncogene 19: 5574–5581 (2000). 3. Scheidegger, K.J., et al., J. Biol. Chem. 275: 38,921–38,928 (2000). 4. Hernandez-Sanchez, C., et al., J. Biol. Chem. 270: 29,176–29,181 (1995). 5. Lopaczynski, W., et al., Biochem. Biophys. Res. Commun. 279: 955–960 (2000). 6. Baserga, R., et al., Exp. Cell Res. 253: 1–6 (1999). 7. White, M.F., et al., J. Biol. Chem. 260: 9470–9478 (1985). 8. White, M.F., et al., J. Biol. Chem. 263: 2969–2980 (1988).

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