antibody

Insulin-Like Growth Factor I Receptor, beta, phosphorylated (Tyr1135) (IGF1Rb)

I7661-21M2

20 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

10E33(DA7A8)

Insulin-Like Growth Factor I Receptor, beta, phosphorylated (Tyr1135) (IGF1Rb)

rabbit

Mab

Phosphorylated

Antibodies

20 ul

10E33(DA7A8)

IgG

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine auto-phosphorylation of the insulin receptor is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Tyr1135 of human IGF-I receptor beta.

Recongnizes endogenous levels of human IGF-I receptor only when phosphorylated at Tyr1135. Cross-reacts with Tyr1150 of insulin receptor and may also cross-react with other overexpressed related tyrosine-phosphorylated tyrosine kinases. Species crossreactivity: mouse and rat

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E33(DA7A8) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr1135 of human IGF-I receptor beta. Specificity: Recongnizes endogenous levels of human IGF-I receptor only when phosphorylated at Tyr1135. Cross-reacts with Tyr1150 of insulin receptor and may also cross-react with other overexpressed related tyrosine-phosphorylated tyrosine kinases. Species crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050–1093, 2. Baserga, R. et al. (2000) Oncogene 19, 5574–5581, 3. Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921–38928, 4. Hernandez-Sanchez, C. et al. (1995) J. Biol. Chem. 270, 29176–29181, 5. Lopaczynski, W. et al. (2000) Biochem. Biophys. Res. Commun. 279, 955–960, 6. Baserga, R. et al. (1999) Exp. Cell Res. 253, 1–6, 7. White, M.F. et al. (1985) J. Biol. Chem. 260, 9470–9478, 8. White, M.F. et al. (1988) J. Biol. Chem. 263, 2969–2980.

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