Insulin | US Biological I7660-25A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Insulin

catalog :

I7660-25A

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

I7660-25A

Product wo Prefix :

Insulin

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Glucose homeostasis is regulated by hormones. Elevations in blood glucose levels during feeding stimulates insulin release from pancreatic beta cells through a glucose sensing pathway. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined by a disulfide bond to form insulin, and the central part of the precursor molecule is cleaved and released as C-peptide. Insulin stimulates glucose uptake from blood into the skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:100 Immunofluorescence (Frozen): 1:100 Immunohistochemistry (Paraffin): 1:100. Antigen retrieval: heat in citrate buffer. Dilute antibody in TBST/5% normal goat serum. Flow Cytometry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to the sequence of human insulin.

Specificity :

Recognizes endogenous levels of total human insulin protein. Species Crossreactivity: mouse and rat

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the sequence of human insulin. Specificity: Recognizes endogenous levels of total human insulin protein. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IC IF IHC

Storage Temperature :

-20°C

Reference :

1. Straub, S.G. & Sharp, G.W., Diabetes Metab. Res. Rev. 18: 451–463 (2002). 2. Concannon, P., et al., Nat. Genet. 19: 292–296 (1998).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/30/I7660-25A_1.jpg

Picture4 Text :

Confocal immunofluorescent analysis of beta-TC-6 and HeLa cells using I7660-25A (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/30/I7660-25A_5.jpg

Picture5 Text :

Flow Cytometric analysis of untreated b-TC cells using I7660-25A (blue) compared to a nonspecific negative control antibody (red).