Immunoglobulin Superfamily Member 4C (IGSF4C, Nectin-Like 4, TSLC1-Like Protein 2, TSLL2) | US Biological I7502-B70 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Immunoglobulin Superfamily Member 4C (IGSF4C, Nectin-Like 4, TSLC1-Like Protein 2, TSLL2)

catalog :

I7502-B70

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

I7502-B70

Product wo Prefix :

Immunoglobulin Superfamily Member 4C (IGSF4C, Nectin-Like 4, TSLC1-Like Protein 2, TSLL2)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

IGSF4C (Immunoglobulin Superfamily member 4C), also known as Nectin-like 4 (Necl-4) and TSLL2, is a 48-55kD type I transmembrane glycoprotein that is a member of the nectin-like family of IGSF molecules. It is expressed in the brain, kidney, bladder and prostate. IGSF4C forms cis homophilic dimers on cell surfaces and mediates cell adhesion through homophilic trans-interactions in a Ca 2+/Mg 2+-independent manner. IGSF4C may also be a tumor-suppressor candidate in human prostate cancer. The mature 364 amino acid (aa) molecule contains a 299 aa extracellular domain (ECD) (aa 25-323) with three Ig-like motifs and a short cytoplasmic domain. There is at least one potential splice variant, which shows an insertion of 15 aa after Tyr309. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 50ug/ml. Add 10ul of diluted antibody to 1-5x10e5 cells (max. total volume 200ul). ELISA: 0.5-1ug/ml; Detection limit for recombinant human IGSF4C is ~0.3ng/well. Western Blot: 0.1-0.2ug/ml; Detection limit for recombinant human IGSF4C is ~5ng/lane (reducing and non-reducing conditions). Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1903-12S, IgG, H&L (PE) Donkey x Goat I1903-30A, IgG, H&L, X-Adsorbed (FITC) Rabbit x Goat Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol in PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

NS0-derived recombinant human IGSF4C extracellular domain (aa25-323) Accession # NP_660339

Specificity :

Recognizes human lGSF4C. In direct ELISAs and Western Blots, shows ~10% crossreactivity with recombinant human IGSF4B and less than 1% crossreactivity with recombinant human IGSF4D. Species Sequence Homology: Canine, mouse - 99%.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

Concentration :

~0.2mg/ml (after reconstitution)

Desc2 :

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Immunogen: NS0-derived recombinant human IGSF4C extracellular domain (aa25-323) Accession # NP_660339 Specificity: Recognizes human lGSF4C. In direct ELISAs and Western Blots, shows ~10% crossreactivity with recombinant human IGSF4B and less than 1% crossreactivity with recombinant human IGSF4D. Species Sequence Homology: Canine, mouse - 99%. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC WB

Storage Temperature :

-20°C