antibody

Immunoglobulin-Like Transcript 4 (ILT4, CD85d) (PE)

I7502-80A

100Tests

monoclonal

mouse

PE

7H14

Immunoglobulin-Like Transcript 4 (ILT4, CD85d) (PE)

mouse

Mab

PE

Antibodies

100Tests

7H14

IgG2a

The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1-3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex that also includes the genes encoding KIRs (4). With the exception of ILT6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains, which allows association with Fcy R, an immunoreceptor tyrosine-based activation motif (ITAM)-bearing signal adapter protein (1-3). ILT-4, also known as Leukocyte Immunoglobulin-like Receptor 2 (LIR-2) and Leukocyte Immunoglobulin-Like Receptor subfamily B, member 2 (LILRB2), is an Ig superfamily inhibitory receptor expressed primarily on monocytes, macrophages, and dendritic cells (DC). Binding of HLA-G to ILT4 on DC alters DC maturation, rendering them tolerogenic. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul, labels up to 1x10e6 human peripheral clood mononuclear cells (PBMCs) Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Hu

Recombinant protein corresponding to Gly24­-His458 of human ILT4 expressed in NS0 cells.

Recognizes human Immunoglobulin-Like Transcript 4. Does not crossreact with rhILT1, rhILT2, rhILT3, rhILT6, rhILT7, rhILT11, rhLIR6 or rhLIR8.

Purified by Protein A affinity chromatography from hybridoma culture supernatant

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).

~0.025mg/ml

Product Type: Mab Isotype: IgG2a Clone No: 7H14 Host: mouse Source: human Concentration: ~0.025mg/ml Form: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Purity: Purified by Protein A affinity chromatography from hybridoma culture supernatant Immunogen: Recombinant protein corresponding to Gly24­-His458 of human ILT4 expressed in NS0 cells. Specificity: Recognizes human Immunoglobulin-Like Transcript 4. Does not crossreact with rhILT1, rhILT2, rhILT3, rhILT6, rhILT7, rhILT11, rhLIR6 or rhLIR8. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC

4°C Do not freeze

1. Allen, D. et al., 2000, Immunobiol. 202:34. 2. Colonna, M. et al., 1999, J. Leukocyte Biol. 66:375. 3. Borges, L. and D. Cosman, 2000, Cytokine Growth Factor Rev. 11:209.