ILK1 (Integrin Linked Kinase) | US Biological I5005-05 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

ILK1 (Integrin Linked Kinase)

catalog :

I5005-05

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

I5005-05

Product wo Prefix :

ILK1 (Integrin Linked Kinase)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extra-cellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt on serine 473, GSK-3 on serine 9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved serine 343 residue in the activation loop plays a key role in the activation of ILK1 (2). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the C-terminus of human ILK1.

Specificity :

Recognizes endogenous levels of total human ILK1 at ~51kD. May crossreact with ILK2. Species Crossreactivity: mouse, rat, monkey and bovine

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the C-terminus of human ILK1. Specificity: Recognizes endogenous levels of total human ILK1 at ~51kD. May crossreact with ILK2. Species Crossreactivity: mouse, rat, monkey and bovine Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC WB

Storage Temperature :

-20°C

Reference :

1. Wu, C. and Dedhar, S. (2000) J. Biol. Chem. 155, 505–510. 2. Persad, S. et al. (2001) J. Biol. Chem. 276, 27462–27469. 3 Persad, S. et al. (2000) J. Cell Biol. 153, 1161–1173. 4. Deng, J.T. et al. (2001) J. Biol. Chem. 276, 16365–16373. 5. Yamaji, S. et al. (2001) J. Cell Biol. 153, 1251–1264. 6. Morimoto, A.M. et al. (2000) Oncogene 19, 200–209.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/10/I5005-05_1.jpg