antibody

IKK gamma (IKK3, NEMO, FIP3, IKKAP1, IkappaB Kinase gamma, IKKg)

I3000-36C

100 ul

monoclonal

mouse

nonconjugated

5i119(DA10-12)

IKK gamma (IKK3, NEMO, FIP3, IKKAP1, IkappaB Kinase gamma, IKKg)

mouse

Mab

Antibodies

100 ul

5i119(DA10-12)

IgG1

The NFkB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins (1-3). Most agents that activate NFkB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKa and IKKb serve as the catalytic subunits of the kinase. IKKg serves as its regulatory subunit (8-9). Activation of IKK depends on phosphorylation; serines 177 and 181 in the activation loop of IKKb (176 and 180 in IKKa) are the specific sites whose phosphorylation causes a conformational change that results in kinase activation (10-13). Activation of the NF-kB pathway by the T-cell lymphotrophic virus Tax protein or by TNF-a treatment leads to IKKb-dependent phosphorylation of human IKKg, primarily at Ser376 (14). In mice, mutation of the orthologous residue (Ser369) to alanine leads to enhanced IKKg-mediated stimlulation of IKKb kinase activity (15). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4ºC with gentle shaking overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Rt

Full length human GSTIKK g protein.

Recognizes endogenous levels of total human IKK gamma protein at ~50kD. Species Crossreactivity: rat

Ascites

Supplied in as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 5i119(DA10-12) Host: mouse Source: human Concentration: Not determined Form: Supplied in as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Ascites Immunogen: Full length human GSTIKK g protein. Specificity: Recognizes endogenous levels of total human IKK gamma protein at ~50kD. Species Crossreactivity: rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. 2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. 3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. 4. Brown, K. et al. (1995) Science 267, 1485-8. 5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. 6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7. Chen, Z.J. et al. (1996) Cell 84, 853-62. 8. Zandi, E. et al. (1997) Cell 91, 243-52. 9. Karin, M. (1999) Oncogene 18, 6867-74. 10. DiDonato, J.A. et al. (1997) Nature 388, 548-54. 11. Mercurio, F. et al. (1997) Science 278, 860-6. 12. Johnson, L.N. et al. (1996) Cell 85, 149-58. 13. Delhase, M. et al. (1999) Science 284, 309-13. 14. Carter, R. S. et al. (2003) 278, 19642-19648. 15. Prajapati, S. and Gaynor, R.B. (2002) J. Biol. Chem. 277, 24331-24339.

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