antibody

IkB alpha, phosphorylated (Ser32/36)

I2500-06A

100 ul

monoclonal

mouse

nonconjugated

phosphorylated

3A32(5A5)

IkB alpha, phosphorylated (Ser32/36)

mouse

Mab

Phosphorylated

Antibodies

100 ul

3A32(5A5)

IgG1

The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins (1-3). Activation occurs via phosphorylation of IkB-alpha at Ser32 and Ser36, resulting in the release and nuclear translocation of active NF-kappaB. IkappB-alpha phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals, including inflammatory cytokines, growth factors and chemokines. Phosphorylation of IkappB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin followed by proteasome-mediated degradation of IkB, resulting in the release of active NF-kappaB. Kinases that phosphorylate IkB at these activating sites have been identified. Because phosphorylation of IkB-alpha at Ser32 is essential for release of active NF-kappaB, phosphorylation at this site is an excellent marker of NF-kappaB activation. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted I2500-06A in TBS, 5% nonfat dry milk, 0.1% Tween-20 overnight at 4°C with gentle shaking. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IkappaB-alpha. Species Sequence Homology: porcine, bovine, canine and guinea pig

Recognizes endogenous levels of human IkappaB-alpha only when phosphorylated at Ser32/36 at 40kD. Species Crossreactivity: mouse, rat and monkey

Ascites

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG1 Clone No: 3A32(5A5) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IkappaB-alpha. Species Sequence Homology: porcine, bovine, canine and guinea pig Specificity: Recognizes endogenous levels of human IkappaB-alpha only when phosphorylated at Ser32/36 at 40kD. Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-546. 2. Beg, A.A. et al. (1993) Genes Dev. 7, 2064-2070. 3. Finco, T.S. et al. (1994) PNAS USA 91, 11884-11888. 4. Brown, K. et al. (1995) Science 267, 1485-1488. 5. Brockman, J.A. et al. (1995) Mol. Cell. Biol. 15, 2809-2818. 6. Traenckner, E.B. et al. (1995) EMBO J. 14, 2876-2883. 7. Chen, Z.J. et al. (1996) Cell 84, 853-862. 8. Karin, M., Ben-Neriah, Y. (2000) Annu. Rev. Immunol. 18, 621-663. 9. Howe, C.J. et al. (2002) J. Biol. Chem. 277, 30469-30476. 10. Howe, C.L., et al.: Precipitation of the CaM-kinase in hydrogen peroxide-induced Ikb phosphorylation in human T lymphocytes. J. Biol. Chem. 277: 30469-30476 (2002).

https://usbio-images.r.worldssl.net/prodimages/37/I2500-06A_1.pct