antibody

IkB alpha

I2500-01

100 ul

polyclonal

domestic rabbit

nonconjugated

IkB alpha

rabbit

Pab

Antibodies

100 ul

IgG

The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins (1-3). Activation occurs via phosphorylation of IkappaB-alpha at Ser32 and Ser36, resulting in the release and nuclear translocation of active NF-kappaB (3-7). IkappaB-alpha phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals, including inflammatory cytokines, growth factors and chemokines. Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin followed by proteasome-mediated degradation of IkappaB, resulting in the release of active NF-kappaB. Kinases that phosphorylate IkappaB at these activating sites have been identified (8). Because phosphorylation of IkappaB-alpha at Ser32 is essential for release of active NF-kappaB, phosphorylation at this site is an excellent marker of NF-kappaB activation (1-3). Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Bo Ca Hu Mk Mo Po Rt

Synthetic peptide derived from the sequence suurounding Arg29 of human IkappaB-alpha (KLH coupled).

Recognizes endogenous levels of total human IkappaB-alpha protein. Species Crossreactivity: mouse, rat, monkey, bovine, canine and porcine.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide derived from the sequence suurounding Arg29 of human IkappaB-alpha (KLH coupled). Specificity: Recognizes endogenous levels of total human IkappaB-alpha protein. Species Crossreactivity: mouse, rat, monkey, bovine, canine and porcine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Baeuerle, P.A. and Baltimore, D., Science 242: 540-546 (1988). 2. Beg, A.A., et al., Genes Dev. 7: 2064-2070 (1993). 3. Finco, T.S., et al., Proc. Natl. Acad. Sci. USA 91: 11,884-11,888 (1994). 4. Brown, K., et al., Science 26: 1485-1488 (1995). 5. Brockman, J.A., et al., Mol. Cell. Biol. 15: 2809-2818 (1995). 6. Traenckner, E.B., et al., EMBO J. 14: 2876-2883 (1995). 7. Chen, Z.J., et al., Cell 84: 853-862 (1996). 8. Karin, M. and Ben-Neriah, Y., Annu. Rev. Immunol. 18: 621-663 (2000). 9. Bonnard, M., et al., EMBO J. 19: 4976-4985 (2000). 10. Burns, K., et al., Nat. Cell Biol. 2: 346-351 (2000). 11. Delehedde, M., et al., J. Biol. Chem. 275: 33,905-33,910 (2000). 12. Hoeflich, K.P., et al., Nature 86-90 (2000).

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