Iduronidase, a-L- (alpha-L-Iduronidase, IDUA) | US Biological I0706-94B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Iduronidase, a-L- (alpha-L-Iduronidase, IDUA)

catalog :

I0706-94B

quantity :

100 ug

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

I0706-94B

Product wo Prefix :

Iduronidase, a-L- (alpha-L-Iduronidase, IDUA)

Host :

sheep

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Alpha-L-Iduronidase (IDUA) is a ubiquitously expressed lysosomal glycosyl hydrolase. It hydrolyzes the unsulfated terminal alpha-L-Iduronic acid residues in glycosaminoglycans including dermatan sulfate and heparin sulfate. Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis. Applications: Suitable for use in Direct ELISA, Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Immunocytochemistry: 5-15ug/ml; immersion fixed HepG2 human hepatocellular carcinoma cell line Immunoprecipitation: 25ug/ml; conditioned cell culture medium spiked with recombinant human IDUA. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Ala26-Pro653 from human alpha-L-Iduronidase/IDUA, expressed in NSO cells.

Specificity :

Recognizes human alpha-L-Iduronidase/IDUA.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

Concentration :

~0.2mg/ml (after reconstitutoin)

Desc2 :

Product Type: Pab Isotype: IgG Host: sheep Source: human Concentration: ~0.2mg/ml (after reconstitutoin) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Immunogen: Recombinant protein corresponding to Ala26-Pro653 from human alpha-L-Iduronidase/IDUA, expressed in NSO cells. Specificity: Recognizes human alpha-L-Iduronidase/IDUA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IC IP WB

Storage Temperature :

-20°C

Reference :

1.Scott, H.S. et al. (1995) Hum. Mutat. 6:288. 2.Wraith, J.E. (2005) Expert Opin. Pharmacother. 6:489.