antibody

Histone H4

H5110-18L

100 ul

monoclonal

mouse

nonconjugated

9B151(L64C1)

Histone H4

mouse

Mab

Antibodies

100 ul

9B151(L64C1)

IgG

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20. Histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:300 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide correponding to the N-terminal sequence of human histone H4. Species Sequence Homology: bovine, D. melanogaster, Xenopus, equine and C. elegans.

Recognizes endogenous levels of total human histone H4 protein. Does not cross-react with other histones. Species Crossreactvity: mouse, rat, monkey and zebrafish.

Ascites

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG Clone No: 9B151(L64C1) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide correponding to the N-terminal sequence of human histone H4. Species Sequence Homology: bovine, D. melanogaster, Xenopus, equine and C. elegans. Specificity: Recognizes endogenous levels of total human histone H4 protein. Does not cross-react with other histones. Species Crossreactvity: mouse, rat, monkey and zebrafish. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IHC WB

-20°C

Workman, J.L. and Kingston, R.E. (1998) Annu. Rev. Biochem. 67, 545-579.Hansen, J.C. et al. (1998) Biochemistry 37, 17637-17641.Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-45.Cheung, P. et al. (2000) Cell 103, 263-271.Bernstein, B.E. and Schreiber, S.L. (2002) Chem. Biol. 9, 1167-1173.Jaskelioff, M. and Peterson, C.L. (2003) Nat. Cell Biol. 5, 395-399.Thorne, A.W. et al. (1990) Eur. J. Biochem. 193, 701-713.Hendzel, M.J. et al. (1997) Chromosoma 106, 348-360.Goto, H. et al. (1999) J. Biol. Chem. 274, 25543-25549.Preuss, U. et al. (2003) Nucleic Acids Res. 31, 878-885.Dai, J. et al. (2005) Genes Dev. 19, 472-488.

https://usbio-images.r.worldssl.net/prodimages/6/H5110-18L_1.jpg