antibody

Histone H3

H5110-12M8

100 ul

monoclonal

domestic rabbit

nonconjugated

10E29(D1H2)

Histone H3

rabbit

Mab

Antibodies

100 ul

10E29(D1H2)

IgG

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11). Applications: Suitable for use in Western Blot, Immunohistochemistry (P) and Immunofluorescence (IF-IC). Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (P): 1:400 Unmasking buffer: Citrate Immunofluorescence (IF-IC): 1:200 IF Protocol: Methanol Permeabilization required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to the C-terminus of the human histone H3 protein. Species Sequence Homology: bovine, melanogaster, xenopus and zebrafish.

Recognizes endogenous levels of total histone H3. Species crossreactivity: mouse, rat and monkey.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, ≤0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E29(D1H2) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, ≤0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the C-terminus of the human histone H3 protein. Species Sequence Homology: bovine, melanogaster, xenopus and zebrafish. Specificity: Recognizes endogenous levels of total histone H3. Species crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IC IF WB

-20°C

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79, 2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41, 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5, 4. Cheung, P. et al. (2000) Cell 103, 263-71, 5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73, 6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9, 7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13, 8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60, 9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9, 10.Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85, 11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

https://usbio-images.r.worldssl.net/prodimages/27/H5110-12M8_1.jpg