Histone H3 (Histone H3.3, H3 Histone Family 3A, H3F3A, H3F3B) (HRP) | US Biological H5110-12M5 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Histone H3 (Histone H3.3, H3 Histone Family 3A, H3F3A, H3F3B) (HRP)

catalog :

H5110-12M5

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

HRP

clone name :

10E27(3H1)

reactivity :

human

product information

Catalog Number :

H5110-12M5

Product wo Prefix :

Histone H3 (Histone H3.3, H3 Histone Family 3A, H3F3A, H3F3B) (HRP)

Host :

rabbit

Product Type :

Mab

Conjugate :

HRP

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

10E27(3H1)

Isotype :

IgG

Desc1 :

Modulation of chromatin structure plays an important role in the regulation of transcription in eukary-otes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Ca Hm Hu Mk Mo Po Rt Ze

Immunogen :

Synthetic peptide corresponding to the C-terminal residues of human histone H3.

Specificity :

Recongnizes endogenous levels of human total histone H3 protein. Species crossreactivity: mouse, rat, monkey, D. melanogaster, canine, porcine, hamster, bovine, zebrafish. Does not cross-react with other proteins.

Purity :

Purified

Form :

Supplied as liquid in PBS, pH 7.4, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP).

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 10E27(3H1) Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in PBS, pH 7.4, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP). Purity: Purified Immunogen: Synthetic peptide corresponding to the C-terminal residues of human histone H3. Specificity: Recongnizes endogenous levels of human total histone H3 protein. Species crossreactivity: mouse, rat, monkey, D. melanogaster, canine, porcine, hamster, bovine, zebrafish. Does not cross-react with other proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79, 2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41, 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5, 4. Cheung, P. et al. (2000) Cell 103, 263-71, 5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73, 6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9, 7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13, 8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60, 9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9, 10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85, 11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/47/H5110-12M5_1.jpg