antibody

Histone H2A1.2, Macro

H5110-08A2

100 ul

polyclonal

domestic rabbit

nonconjugated

Histone H2A1.2, Macro

rabbit

Pab

Antibodies

100 ul

IgG

Histone macroH2A1 and macroH2A2 comprise a family of variant histone H2A proteins. MacroH2A1 exists as two distinct isoforms due to alternative splicing of a single gene; macroH2A1.1 levels accumulate throughout differentiation and development while macroH2A1.2 shows a constant level of expression (1). MacroH2A1 and macroH2A2 are encoded by completely distinct genes located on separate chromosomes (2,3). Both macroH2A1 and macroH2A2 proteins contain an amino-terminal histone-like region with 64% sequence identity to canonical histone H2A, in addition to a carboxy-terminal “macro” domain (1-3). MacroH2A1 and macroH2A2 are enriched in facultative heterochromatin, including inactivated X chromosomes in mammalian females and senescence-associated heterochromatin foci (2-5). Both act to repress gene transcription by inhibiting the binding of transcription factors to chromatin, the acetylation of histones by p300, and the chromatin-remodeling activities of SWI/SNF and ACF (6,7). The macro domain of macroH2A1.1 binds to ADP-ribose and functions to recruit macroH2A1.1 to activated PARP at sites of DNA damage, where it mediates chromatin rearrangements to lo- cally regulate the DNA damage response (8). MacroH2A1.2 and macroH2A2 do not bind poly-ADP-ribose and are not recruited to sites of activated PARP (8). Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:200. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to the human MacroH2A1.2 protein (MacroH2A1, isoform 2). Species Sequence Homology: chicken, bovine.

Recognizes endogenous levels of the core histone MacroH2A1.2 protein (MacroH2A1, isoform 2). Does not cross-react with MacroH2A1.1 (MacroH2A1, isoform 1), MacroH2A2 or histone H2A. Species crossreactivity: mouse, rat, monkey.

Purified by Protein A and peptide affinity chromatography.

Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the human MacroH2A1.2 protein (MacroH2A1, isoform 2). Species Sequence Homology: chicken, bovine. Specificity: Recognizes endogenous levels of the core histone MacroH2A1.2 protein (MacroH2A1, isoform 2). Does not cross-react with MacroH2A1.1 (MacroH2A1, isoform 1), MacroH2A2 or histone H2A. Species crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF WB

-20°C

1. Pehrson, J.R. et al. (1997) J Cell Biochem 65, 107-13, 2. Chadwick, B.P. and Willard, H.F. (2001) Hum Mol Genet 10, 1101-13, 3. Costanzi, C. and Pehrson, J.R. (2001) J Biol Chem 276, 21776-84, 4. Costanzi, C. and Pehrson, J.R. (1998) Nature 393, 599-601, 5. Zhang, R. et al. (2005) Dev Cell 8, 19-30, 6. Angelov, D. et al. (2003) Mol Cell 11, 1033-41, 7. Doyen, C.M. et al. (2006) Mol Cell Biol 26, 1156-64, 8. Timinszky, G. et al. (2009) Nat Struct Mol Biol 16, 923-9.

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