antibody

Histone H3, phosphorylated (Ser10) (Alexa Fluor® 488) discontinued

H5110-04B

100 ul

polyclonal

domestic rabbit

AF488

phosphorylated

Histone H3, phosphorylated (Ser10) (Alexa Fluor® 488) discontinued

rabbit

Pab

Alexa Fluor®488

Phosphorylated

Antibodies

100 ul

IgG

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8). Applications: Suitable for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Immunofluorescence (IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: Store at 4°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Product sensitive to light.

Hu Mo

Synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3 (KLH).

Recognizes endogenous levels of human histone H3 only when phosphorylated at serine 10 (Mr 17kD). Does not crossreact with other phosphorylated histones or with acetylated histones. Species Crossreactivity: mouse.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 488 (F/P ratio: 2-6).

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 488 (F/P ratio: 2-6). Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3 (KLH). Specificity: Recognizes endogenous levels of human histone H3 only when phosphorylated at serine 10 (Mr 17kD). Does not crossreact with other phosphorylated histones or with acetylated histones. Species Crossreactivity: mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IC IF

4°C Do Not Freeze

1. Workman, J.L.,Kingston, R.E., Annu. Rev. Biochem. 67: 545-579 (1998). 2. Hansen, J.C., et al., Biochemistry 37: 17637-17641 (1998). 3. Strahl, B.D., Allis, C.D., Nature 403: 41-45 (2000). 4. Cheung, P., et al., Cell 103: 263-271 (2000). 5. Bernstein, B.E,. Schreiber, S.L., Chem. Biol. 9: 1167-1173 (2002). 6. Jaskelioff, M. , Peterson, C.L., Nat. Cell. Biol. 5: 395-399 (2003). 7. Thorne, A.W., et al., Eur. J. Biochem. 193: 701-713 (1990). 8. Hendzel, M.J., et al., Chromosoma 106: 348-360 (1997).

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