Histamine | US Biological H5080-04 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Histamine

catalog :

H5080-04

quantity :

50 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

H5080-04

Product wo Prefix :

Histamine

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

50 ul

Isotype :

IgG

Desc1 :

Histamine is a neurotransmitter in the central nervous system a well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach. Applications: Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:1500-1:2000 (PAP). Immunohistochemistry (Paraffin sections): 1:1500-1:2000 (PAP). Requires HIER. Immunofluorescence: 1:300-1:600 with overnight incubation at 4°C. Optimal dilutions to be determined by the researcher. Recommended Positive Control: Cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin-sections (vapor fixed in diethylpyrocarbonate; DEPC) of rat stomach. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Rt

Immunogen :

Histamine, conjugated to HSA

Specificity :

Recognizes Histamine. Absorption with 10-100ug histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagon and histidine do not. Species Crossreactivity: human and rat

Purity :

Serum

Form :

Supplied as a lyophilized powder. Reconstitute with sterile ddH2O. Further dilutions should be made in PBS, 1% BSA, 0.09% sodium azide.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: Not Determined Form: Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O. Further dilutions should be made in PBS, 1% BSA, 0.09% sodium azide. Purity: Serum Immunogen: Histamine, conjugated to HSA Specificity: Recognizes Histamine. Absorption with 10-100ug histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagon and histidine do not. Species Crossreactivity: human and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF IHC

Storage Temperature :

-20°C

Reference :

1. Hakansson R et al. Histamine in endocrine cells in the stomach. A survey of several species using a panel of histamine antibodies. Histochemistry 86, 5-17 (1986) 2. Axelson J et al: Enhanced hyperplasia of gastric enterochromaffinlike cells in response to omeprazole-evoked hypergastrinemia in rats with portacaval shunts. Gastroenterology 99, 635-640 (1990) 3. Lönroth H et al: Histamine containing endocrine cells in the human stomach. Gut 31, 383-388 (1990) 4. Johansson O et al. Histamine immunohistochemistry: a new and highly sensitive method for studying cutaneous mast cells. Histochem J 24, 283-187 (1992)