antibody

HIPK2 (Homeodomain Interacting Protein Kinase 2, hHIPk2)

H4065-10C

100 ul

polyclonal

domestic rabbit

nonconjugated

HIPK2 (Homeodomain Interacting Protein Kinase 2, hHIPk2)

rabbit

Pab

Antibodies

100 ul

IgG

Members of the homeodomain-interacting protein kinase (HIPK1-4) family of serine/threonine kinases regulate gene transcription with effects on cell proliferation, differentiation, and apoptosis (1-3). HIPK1-3 are nuclear proteins that were originally described as co-repressors for homeobox transcription factors (1). HIPK proteins can interact with and/or phosphorylate many transcriptional regulators (4). HIPK2 activated in response to DNA damage, including UV radiation and chemotherapeutic drugs, phosphorylates p53 at Ser46 to promote the transcription of pro-apoptotic p53 target genes (5-7). In addition, HIPK2 interacts with a number of transcription factors that control developmental processes, tumor suppression and apoptosis (4). The kinase is regulated by both sumoylation (8) and ubiquitination (9,10). Ubiquitination and subsequent degradation of HIPK2 is inhibited by DNA damaging agents. Caspase-dependent cleavage of HIPK2 removes the inhibitory domain and results in enhanced HIPK2 activity (11). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide corresponding to residues surrounding Gln1045 of human HIPK2.

Recognizes endogenous levels of total human HIPK2. Species crossreactivity: mouse and rat

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Gln1045 of human HIPK2. Specificity: Recognizes endogenous levels of total human HIPK2. Species crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Kim, Y.H. et al. (1998) J Biol Chem 273, 25875-9. 2. Rochat-Steiner, V. et al. (2000) J. Exp. Med. 192, 1165-1174. 3. Arai, S. et al. (2007) FEBS Lett 581, 5649-57. 4. Rinaldo, C. et al. (2007) Biochem Cell Biol 85, 411-8. 5. Hofmann TG et al. (2002) Nat Cell Biol 4, 1–10. 6. Gresko E et al. (2006) EMBO J 25, 1883–94. 7. Di Stefano, V. et al. (2004) Exp Cell Res 293, 311-20. 8. Kim, Y.H. et al. (1999) Proc Natl Acad Sci U S A 96, 12350-5. 9. Choi, D.W. et al. (2008) J Biol Chem 283, 4682-9. 10. Winter, M. et al. (2008) Nat Cell Biol 10, 812-24. 11. D'Orazi, G. et al. (2002) Nat Cell Biol 4, 11-9.

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