antibody

Heme Oxygenase 1 (HO-1, HSP32, Heat Shock Protein 32)

H1847-69

25 ug

monoclonal

mouse

nonconjugated

4E134 (HO-1-1)

Heme Oxygenase 1 (HO-1, HSP32, Heat Shock Protein 32)

mouse

Mab

Antibodies

25 ug

4E134 (HO-1-1)

IgG1,k

Heme oxygenase-1 (HO-1,) also known as HSP32, is the inducible isoform of heme oxygenase which catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, iron and biliverdin that is immediately reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; “free” iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5’-or 3’-UTRs of the mRNAs. To date, three heme oxygenase isoforms, HO-1, HO-2 and HO-3, have been identified. HO-1 or HSP32, a major heat shock/stress response protein, is ubiquitous and its mRNA as well as its activity can be increased several-fold by heme, other metalloporphyrins, transition metals and stimuli that induce cellular stress. The 5’-untranslated region (UTR) of HO-1 has several consensus regulatory elements which include sites for activator protein 1 (AP-1), metal responsive element (MRE), oncogene c-myc/max heterodimer binding site (Myc/Max), antioxidant response element (ARE) and GC box binding (Sp1) (1). HO-1 expression has been shown to increase in benign postatic hyperplasia (BPH) and malignant prostate tissue suggesting a role for this stress protein in the pathogenesis of BPH and prostate cancer (2). Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:250 (ECL and Colormetric). Microsomal preparations are recommended for optimal detection of HO-1. Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Recommended Positive Control: Recombinant Human Heme-Oxygenase-1 Protein Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Ca Hu Mo Rt

Synthetic peptide corresponding to a sequence near the amino terminus of human HO-1 (Hsp32).

Recognizes human HO-1 at ~32kD. Does not crossreact with HO-2. Species Crossreactivity: mouse, rat, bovine and canine.

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.

~1mg/ml

Product Type: Mab Isotype: IgG1,k Clone No: 4E134 (HO-1-1) Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol. Purity: Purified by Protein G affinity chromatography. Immunogen: Synthetic peptide corresponding to a sequence near the amino terminus of human HO-1 (Hsp32). Specificity: Recognizes human HO-1 at ~32kD. Does not crossreact with HO-2. Species Crossreactivity: mouse, rat, bovine and canine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC WB

-20°C

1. Elbirt, K.K. and Bonkovsky, H.L., Proc. Assoc. Am Physicians 111: 438-447 (1999). 2. Maines, M.D. and Abrahamsson, P.A., Urology 47: 727-733 (1996). 3. Foresti, R., et al., Biochem J. 339: 729-736 (1999). 4. Yoshida, T., et al., Eur. J. Biochem. 171: 457-461 (1988). 5. Yoshida, T., et al., Circulation 103: 1695-1701 (2001). 6. Hadjur, S., et al., Blood 98: 1003-1011 (2001). 7. Alcaraz, M.J., et al., Br. J. Pharmacol. 130: 57-64 (2000). 8. Bachelet, M., et al., Allergy 57: 791-797 (2002).

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