Heme Oxygenase 1 (Pro249) (HO-1, HSP32, Heat Shock Protein 2) | US Biological H1847-68H product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Heme Oxygenase 1 (Pro249) (HO-1, HSP32, Heat Shock Protein 2)

catalog :

H1847-68H

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

H1847-68H

Product wo Prefix :

Heme Oxygenase 1 (Pro249) (HO-1, HSP32, Heat Shock Protein 2)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Heme oxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory and cytoprotective functions (2). Heme oxyganse is comprised of two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer disease, endotoxic shock, diabetes, inflammation and neurological disorders (1,5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to amino acid residues surrounding Pro249 of human HO-1 protein.

Specificity :

Recognizes endogenous levels of total human HO-1 protein.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to amino acid residues surrounding Pro249 of human HO-1 protein. Specificity: Recognizes endogenous levels of total human HO-1 protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Abraham, N.G. and Kappas, A. (2008) Pharmacol Rev 60, 79-127, 2. Otterbein, L.E. et al. (2003) Trends Immunol 24, 449-55, 3. Cruse, I. and Maines, M.D. (1988) J Biol Chem 263, 3348-53, 4. Maines, M.D. (1988) FASEB J 2, 2557-68, 5. Schipper, H.M. et al. (2009) Curr Alzheimer Res 6, 424-30.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/15/H1847-68H_1.jpg