Helicobacter pylori | US Biological H1840-14 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Helicobacter pylori

catalog :

H1840-14

quantity :

1 ml

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

H1840-14

Product wo Prefix :

Helicobacter pylori

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

1 ml

Isotype :

IgG

Desc1 :

Helicobacter pylori is a spiral-shaped, Gram-negative rod approximately 0.5x3.0um in size. The catalase-positive organism has 4-6 sheathed flagella attached to one pole which allow for motility. Helicobacter pylori lives in the stomach and duodenum (section of intestine just below stomach). It has a unique way of adapting in the harsh environment of the stomach. The inside of the stomach is bathed in about half a gallon of gastric juice every day. Gastric juice is composed of digestive enzymes and concentrated hydrochloric acid, which can readily tear apart the toughest food or microorganism. Bacteria, viruses, and yesterdays steak dinner are all consumed in this deadly bath of chemicals. It used to be thought that the stomach contained no bacteria and was actually sterile, but Helicobacter pylori changed that. The stomach is protected from its own gastric juice by a thick layer of mucus that covers the stomach lining. Helicobacter pylori takes advantage of this protection by living in the mucus lining. Once H. pylori is safely ensconced in the mucus, it is able to fight the stomach acid that does reach it with an enzyme it possesses called urease. Urease converts urea, of which there is an abundant supply in the stomach (from saliva and gastric juices), into bicarbonate and ammonia, which are strong bases. This creates a cloud of acid neutralizing chemicals around the H. pylori, protecting it from the acid in the stomach. The reaction of urea hydrolysis is important for diagnosis of H. pylori by the breath test. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry It is also suitable for Conjugation purposes. Other applications not tested. Recommended Dilution: Immunofluorescence (formalin-fixed): >1:1000 ELISA: >1:5000 Western Blot: Multiple bands 28-87kD Immunohistochemistry: formalin-fixed Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Whole cell lysate. ATCC strain 43504.

Specificity :

Recognizes Helicobacter pylori. May react with related microorganisms.

Purity :

Purified IgG fraction to a purity of ≥95%.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.

Concentration :

~4-5mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~4-5mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added. Purity: Purified IgG fraction to a purity of ≥95%. Immunogen: Whole cell lysate. ATCC strain 43504. Specificity: Recognizes Helicobacter pylori. May react with related microorganisms. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C