Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60 (D307) | US Biological H1834-25J product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60 (D307)

catalog :

H1834-25J

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

H1834-25J

Product wo Prefix :

Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60 (D307)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). It is now clear that a significant amount of HSP60 is also present in the cytosol of many cells and that it is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8). Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:50 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Dr Hu Mk Mo Rt

Immunogen :

Synthetic peptide surrounding Asp307 of human HSP60.

Specificity :

Recognizes endogenous levels of total human HSP60 protein. Does not cross-react with other HSPs. Species crossreactivity: mouse, rat, monkey, D.melanogaster.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide surrounding Asp307 of human HSP60. Specificity: Recognizes endogenous levels of total human HSP60 protein. Does not cross-react with other HSPs. Species crossreactivity: mouse, rat, monkey, D.melanogaster. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IF WB

Storage Temperature :

-20°C

Reference :

1. Hartl, F.U. (1996) Nature 381, 571–579. 2. Bukau, B. and Horwich, A.L. (1998) Cell 92, 351–366. 3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852–1858. 4. Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279–2283. 5. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931–5938. 6. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51–58. 7. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116–128. 8. Lai, H.C. et al. (2007) Am J Physiol Endocrinol Metab 292, E292-E297.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/12/H1834-25J_1.jpg