antibody

HSP70 (78kD Glucose-regulated Protein, GRP 78, Heat Shock 70kD Protein 5, Immunoglobulin Heavy Chain-binding Protein, BiP, Endoplasmic Reticulum Lumenal Ca(2+)-binding Protein grp78, HSPA5, GRP78)

H1830-97K

100 ug

polyclonal

domestic rabbit

nonconjugated

HSP70 (78kD Glucose-regulated Protein, GRP 78, Heat Shock 70kD Protein 5, Immunoglobulin Heavy Chain-binding Protein, BiP, Endoplasmic Reticulum Lumenal Ca(2+)-binding Protein grp78, HSPA5, GRP78)

rabbit

Pab

Antibodies

100 ug

IgG

Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78kD (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75kD protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell. It should be noted, however, that there is a constitutive level of Hsp70 expression in some cell types such as primates, e.g., human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Hm Hu Mk Mo Rb Rt

Synthetic peptide corresponding to aa559 from human Hsp70.

Recognizes human Hsp70. Species Crossreactivity: mouse, rat, monkey, hamster, rabbit and bovine. Does not cross-react with the constitutive Hsc70.

Purified by affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 30% glycerol.

~0.5mg/ml

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 30% glycerol. Purity: Purified by affinity chromatography. Immunogen: Synthetic peptide corresponding to aa559 from human Hsp70. Specificity: Recognizes human Hsp70. Species Crossreactivity: mouse, rat, monkey, hamster, rabbit and bovine. Does not cross-react with the constitutive Hsc70. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C