antibody

Heat Shock Protein 70 (HSP70)

H1830-95B

50 ug

monoclonal

mouse

nonconjugated

7A1

Heat Shock Protein 70 (HSP70)

mouse

Mab

Antibodies

50 ug

7A1

IgG1

Hsp70 genes encode abundant heat-inducible 70-kD hsps (hsp70s). In most eucaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eucaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot, Immunofluorescence/Immunocytochemistry, Immuno-electron Microscopy and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1ug/ml was sufficient for colorimetric detection of Hsp/Hsc70 in 20ug of heat shocked HeLa lysate using goat anti-mouse IgG:HRP as the secondary antibody. Immunohistochemistry: 1:100 Immunofluorescence (IC): 1:50 Optimal dilutions to be determined by the researcher. Positive Control: BT474 or HeLa cells, breast carcinoma. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Bo Ce Ca Ch Dr Gp Hm Hu Mk Mo Po Rb Rt Sh Xe

Recombinant hsp70/hsc70 (1). Cellular Localization: Nuclear and cytoplasmic. Swiss/Uniprot Accession: P08107

Recognizes 72 and 73kD proteins corresponding to the molecular mass of inducible hsp and hsc70 of SDS PAGE immunoblots. Species Crossreactivity: Human, mouse, rat, bovine, C. elegans, beluga, canine, chicken, Drosophila, fish (carp, Chinook/chum salmon, rainbow trout), guinea pig, hamster, monkey, porcine, plant (cumcumber/pea), rabbit, sheep, Xenopus.

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

~1mg/ml

Product Type: Mab Isotype: IgG1 Clone No: 7A1 Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to human hsp70/hsc70 (1). Cellular Localization: Nuclear and cytoplasmic. Swiss/Uniprot Accession: P08107 Specificity: Recognizes 72 and 73kD proteins corresponding to the molecular mass of inducible hsp and hsc70 of SDS PAGE immunoblots. Species Crossreactivity: Human, mouse, rat, bovine, C. elegans, beluga, canine, chicken, Drosophila, fish (carp, Chinook/chum salmon, rainbow trout), guinea pig, hamster, monkey, porcine, plant (cumcumber/pea), rabbit, sheep, Xenopus. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF IHC IP WB

-20°C

1. Welch, W.J. and Suhan, J.P. (1986) J.Cell Biol. 103: 2035-2050. 2. Boorstein, W. R., Ziegelhoffer, T. & Craig, E. A. (1993), J. Mol. Evol. 38(1) 1-17. 3. Rothman, J. (1989), Cell 59, 591-601. 4. DeLuca-Flaherty et al. (1990), Cell 62, 875-887. 5. Bork, P., Sander, C. & Valencia, A. (1992), Proc. Nut1 Acad. Sci. USA 89, 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Polanonka-Grabowska, R. et. al. (1997) Blood 90: 1516-1526. 8. Schnell, D.J. et. al. (1994) Science 266: 1007-1012. 9. Kabakov, A.E., et. al. (2002) Am. J. Physiol. 283(2): C521-C534. 10. Ricart, J. et. al. (1997) Biochem. J. 324: 635-643. 11. Hang, H. and Fox, M.H. (1995) Cytometry 19(2): 119-125.

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