antibody

Heat Shock Protein 27 (HSP27)

H1830-55

100 ul

monoclonal

mouse

nonconjugated

3A13(G31)

Heat Shock Protein 27 (HSP27)

mouse

Mab

Antibodies

100 ul

3A13(G31)

IgG1

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold in order to confer cellular resistance to the adverse environmental change. HSP27 is also phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as the result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been illustrated that phosphorylation and increased concentration of HSP27 modulate actin polymerization and reorganization (5,6). Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50 Immunocytochemistry (for IF): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk

Full length HSP27 protein. MW of Antigen: 27kD.

Recognizes endogenous levels of total HSP27 protein. Does not crossreact with other heat shock proteins. Species Crossreactivity: Human and monkey.

Ascites

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol and 0.02% sodium azide.

Not Determined

Product Type: Mab Isotype: IgG1 Clone No: 3A13(G31) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol and 0.02% sodium azide. Purity: Ascites. Immunogen: Full length HSP27 protein. MW of Antigen: 27kD. Specificity: Recognizes endogenous levels of total HSP27 protein. Does not crossreact with other heat shock proteins. Species Crossreactivity: Human and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IC IF IHC WB

4°C (-20°C Glycerol)

1. Arrigo, A.P. and Landry, J. (1994) The Biology of Heat Shock Proteins and Molecular Chaperones. Cold Spring Harbor Laboratory Press, NY, 335–373. 2. Landry, J., et al. (1992) J. Biol. Chem. 267: 794–803. 3. Rouse, J., et al. (1994) Cell 78: 1,027–1,037. 4. Rogalla, T., et al. (1999) J. Biol. Chem. 274: 18,947–18,956. 5. Lavoie, J., et al. (1993) J. Biol. Chem. 268: 24,210–24,214. 6. Rousseau, S., et al. (1997) Oncogene 15: 2,169–2,177. 7. Sarkar, D., et al. (2002) Proc. Natl. Acad. Sci. USA 99: 10,054–10,059.

https://usbio-images.r.worldssl.net/prodimages/47/H1830-55_1.jpg

Immunocytochemical staining of HeLa cells, showing cytoplasmic staining, using H1830-55 .