antibody

HDAC1 (Histone Deacetylase 1, HD1, HD-1, HDAC1, HDAC-1, RPD3, RPD3L1, Reduced Potassium Dependency 3 Like-1)

H1827-50U

20 ul

monoclonal

mouse

nonconjugated

10g84(10E2)

HDAC1 (Histone Deacetylase 1, HD1, HD-1, HDAC1, HDAC-1, RPD3, RPD3L1, Reduced Potassium Dependency 3 Like-1)

mouse

Mab

Antibodies

20 ul

10g84(10E2)

IgG1

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate non-histone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I (HDACs 1, 2, 3 and 8) proteins are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9 and 10) are related to yeast Hda1-like proteins and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to the C-terminus of human HDAC1 protein.

Recognizes endogenous levels of total human HDAC1 protein. Does not cross-react with other HDAC proteins. Species crossreactivity: mouse, rat and monkey.

Ascites

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 10g84(10E2) Host: mouse Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide corresponding to the C-terminus of human HDAC1 protein. Specificity: Recognizes endogenous levels of total human HDAC1 protein. Does not cross-react with other HDAC proteins. Species crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. 2. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202. 3. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53. 4. Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16. 5. Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83. 6. Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100. 7. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.

https://usbio-images.r.worldssl.net/prodimages/3/H1827-50U_1.jpg