antibody

HCST (DAP10, Hematopoietic Cell Signal Transducer, DNAX-activation Protein 10, Membrane Protein DAP10, Transmembrane Adapter Protein KAP10, KAP10, PIK3AP, UNQ587/PRO1157)

H1827-45B

100 ug

polyclonal

domestic goat

nonconjugated

human, mouse, rat

HCST (DAP10, Hematopoietic Cell Signal Transducer, DNAX-activation Protein 10, Membrane Protein DAP10, Transmembrane Adapter Protein KAP10, KAP10, PIK3AP, UNQ587/PRO1157)

goat

Pab

Antibodies

100 ug

IgG

Transmembrane adapter protein which associates with NKG2D to form an activation receptor NKG2D-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with NKG2D-DAP10 triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full NKG2D-HCST-mediated activation and ultimate killing of target cells. In NK cells, NKG2D-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. NKG2D-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express NKG2D ligands escape from immune surveillance mediated by NK cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:128,000 Western Blot: 0.2-0.6ug/ml. Detects a band at ~ 40kD in human spleen lysates (calculated MW of 9.5kD). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide corresponding to C-RSPAQEDGKVYIN, from the C-termainal of human HCST. Species Sequence Homology: mouse and rat

Recognizes human HCST. Recognizes isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking the residue E81 (NP_001007470.1).

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

~0.5mg/ml

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-RSPAQEDGKVYIN, from the C-termainal of human HCST. Species Sequence Homology: mouse and rat Specificity: Recognizes human HCST. Recognizes isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking the residue E81 (NP_001007470.1). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Upshaw JL, Arneson LN, Schoon RA, Dick CJ, Billadeau DD, Leibson PJ. NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells. Nat Immunol. 2006 May;7(5):524-32. Epub 2006 Apr 2.

https://usbio-images.r.worldssl.net/prodimages/15/H1827-45B_1.jpg