GRK2 (Beta-adrenergic Receptor Kinase 1, Beta-ARK-1, G-protein Coupled Receptor Kinase 2, ADRBK1, BARK, BARK1) | US Biological G8971-95C product information

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product summary

company name :

US Biological

product type :

antibody

product name :

GRK2 (Beta-adrenergic Receptor Kinase 1, Beta-ARK-1, G-protein Coupled Receptor Kinase 2, ADRBK1, BARK, BARK1)

catalog :

G8971-95C

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

product information

Catalog Number :

G8971-95C

Product wo Prefix :

GRK2 (Beta-adrenergic Receptor Kinase 1, Beta-ARK-1, G-protein Coupled Receptor Kinase 2, ADRBK1, BARK, BARK1)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Beta-adrenergic receptor kinase (ADRBK1), also known as GRK2, phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. ADRBK1 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Heart failure is accompanied by severely impaired beta-adrenergic receptor (beta-AR) function. An important mechanism for the rapid desensitization of beta-AR function is agonist-stimulated receptor phosphorylation by the beta-AR kinase (beta-ARK1), an enzyme known to be elevated in failing human heart tissue. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 0.3-1ug/ml, observed in Human lysates of Burkitt's lymphoma cell line Daudi on ~75-80kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Ca Hu Mo Po Rt

Immunogen :

Synthetic peptide corresponding to C-RDAYREAQQLVQR, from human ADRBK1, at the internal region of the protein (NP_001610.2).

Specificity :

Recognizes human ADRBK1. Species sequence homology: Bovine, canine, mouse, porcine and rat.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-RDAYREAQQLVQR, from human ADRBK1, at the internal region of the protein (NP_001610.2). Specificity: Recognizes human ADRBK1. Species sequence homology: Bovine, canine, mouse, porcine and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Iaccarino G, Barbato E, Cipolletta E, De Amicis V, Margulies KB, Leosco D, Trimarco B, Koch WJ. Elevated myocardial and lymphocyte GRK2 expression and activity in human heart failure. Eur Heart J. 2005 Sep;26(17):1752-8. Epub 2005 Jul 29.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/15/G8971-95C_1.jpg