Green Fluorescent Protein (GFP) (HRP) | US Biological G8965-20 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP) (HRP)

catalog :

G8965-20

quantity :

500 ug

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

1003

product information

Catalog Number :

G8965-20

Product wo Prefix :

Green Fluorescent Protein (GFP) (HRP)

Host :

mouse

Product Type :

Mab

Conjugate :

HRP

Category :

Antibodies

Size1 :

500 ug

Clone # USB :

1003

Isotype :

IgG1,k

Desc1 :

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 246 amino acids. It emits green light (emission maximum at 509nm) when excited with blue or UV light. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (Sandwich or capture): 1:10,000-1:50,000 Western Blot: 1:2000-1:5000 Immunohistochemistry: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Source: Tissue culture supernatant Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyﬁsh Aequorea victoria.

Specificity :

Recognized wild type and recombinant forms of GFP. No reaction is seen against RFP. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti mouse serum.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% Thimerosal. Labeled with Horseradish Peroxidase (HRP).

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 1003 Host: mouse Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% Thimerosal. Labeled with Horseradish Peroxidase (HRP). Purity: Purified by Protein A affinity chromatography. Immunogen: GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyﬁsh Aequorea victoria. Specificity: Recognized wild type and recombinant forms of GFP. No reaction is seen against RFP. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti mouse serum. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C