Green Fluorescent Protein (GFP) (Biotin) | US Biological G8965-15 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP) (Biotin)

catalog :

G8965-15

quantity :

500 ug

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

1002

product information

Catalog Number :

G8965-15

Product wo Prefix :

Green Fluorescent Protein (GFP) (Biotin)

Host :

mouse

Product Type :

Mab

Conjugate :

Biotin

Category :

Antibodies

Size1 :

500 ug

Clone # USB :

1002

Isotype :

IgG1,k

Desc1 :

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids. It emits green light (emission maximum at 509nm) when excited with blue or UV light. Applications: Suitable for use in ELISA, Western Blot, Immunofluroescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 (sandwich or capture). Western Blot: 1:2000-1:10,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyﬁsh Aequorea victoria.

Specificity :

Recognizes wild type and recombinant forms of Green Fluorescent Protein (GFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-mouse serum. Does not recognize enhanced forms of GFP.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 1002 Host: mouse Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Purity: Purified by Protein A affinity chromatography. Immunogen: GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyﬁsh Aequorea victoria. Specificity: Recognizes wild type and recombinant forms of Green Fluorescent Protein (GFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-mouse serum. Does not recognize enhanced forms of GFP. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF IHC WB

Storage Temperature :

-20°C