GFP (Green Fluorescent Protein) | US Biological G8965-12 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

GFP (Green Fluorescent Protein)

catalog :

G8965-12

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

G8965-12

Product wo Prefix :

GFP (Green Fluorescent Protein)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and the demonstration that GFP can be expressed as a functional transgene, GFP has become a unique new tool with exciting applications in cell, developmental and molecular biology. GFP is an ideal fluorescent probe: its fluorescence is not species specific (it has been expressed from bacteria, yeast, plant to mammalian cells); it can fuse with proteins of interest without interfering significantly with their assembly or function. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000 Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

E. coli-expressed full length GFP (green fluorescent protein).

Specificity :

Recognizes wild-type GFP and its variants such as EGFP and EBFP.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 0.05% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: E. coli-expressed full length GFP (green fluorescent protein). Specificity: Recognizes wild-type GFP and its variants such as EGFP and EBFP. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Muller, O. et al, J. Cell Science 116: 1107-1115 (2003). 2. Eldstrom, J. et al., FEBS Letts. 531: 529-537 (2002). 3. Brown, D.D. et al., PNAS 102: 12455-12458 (2005). 4. Tulin, A. et al, Genetics 172; 363-371 (2006).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/48/G8965-12_1.jpg