Green Fluorescent Protein (GFP) (AP) | US Biological G8965-11 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP) (AP)

catalog :

G8965-11

quantity :

1 mg

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

5H5

product information

Catalog Number :

G8965-11

Product wo Prefix :

Green Fluorescent Protein (GFP) (AP)

Host :

mouse

Product Type :

Mab

Conjugate :

Category :

Antibodies

Size1 :

1 mg

Clone # USB :

5H5

Isotype :

IgG1,k

Desc1 :

Applications: Suitable for use in ELISA (sandwich or capture), Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1000 to 1:5000. 1.0ug of G8965-11 in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Calc Crossreactivity :

Immunogen :

GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Specificity :

Recognizes wild type, recombinant and enhanced forms of GFP. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Purity :

Purified by Protein A chromatography.

Form :

Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, 10 mg/ml BSA, (IgG and protease free), 0.01% sodium azide, pH 8.0. Labeled with Alkaline Phosphatase (AP).

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 5H5 Host: mouse Concentration: ~1mg/ml Form: Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, 10 mg/ml BSA, (IgG and protease free), 0.01% sodium azide, pH 8.0. Labeled with Alkaline Phosphatase (AP). Purity: Purified by Protein A chromatography. Immunogen: GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. Specificity: Recognizes wild type, recombinant and enhanced forms of GFP. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

4°C Do Not Freeze

Reference :

1. Avarameas and Ternyrock, Immunochemistry 32: 1175 1971.