Green Fluorescent Protein (GFP) | US Biological G8965-10A product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP)

catalog :

G8965-10A

quantity :

50 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

G8965-10A

Product wo Prefix :

Green Fluorescent Protein (GFP)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

50 ul

Isotype :

IgG

Desc1 :

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells. Applications: Suitable for use in ELISA, Electron Microscopy, Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:40,000 Western Blot: 1:1000-1:3000 Immunofluorescence (IC): 1:10-1:500 Immunohistochemistry: 1:10-1:500 Immunohistochemistry (Paraffin): 1:10-1:500 Immunoprecipitation: 1:10-1:500 Optimal dilution to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Highly purified recombinant (full length) GFP made in E. coli.

Specificity :

Recognizes Green Fluorescent Protein (GFP). Species crossreactivity: all variants of Aequorea victoria GFP; S65T-GFP, RS-GFP, YFP and EGFP.

Purity :

Purified by Immunoaffinity chromatography

Form :

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. Purity: Purified by Immunoaffinity chromatography. Immunogen: Highly purified recombinant (full length) GFP made in E. coli. Specificity: Recognizes Green Fluorescent Protein (GFP). Species crossreactivity: all variants of Aequorea victoria GFP; S65T-GFP, RS-GFP, YFP and EGFP. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1.McCabe, et al. Functional roles for fatty Acylated Amino-terminal domains in subcellular localization. Mol Biol Cell. 10: 3771-3786, 1999. 2.Jasinska, et al. Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and sphingolipid phosphate esters J Biochem. 340: 677-686, 1999. 3. Zhang, et al. Identification of structurally important domains of lipid phosphate phosphatase-1: implications for sites of actionî J Biochem. 345: 181-184, 2000. 4.Mesaeli, N., et al. Calreticulin is essential for cardiac development. J. Cell Biology. 144(5): 857-68, 1999.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/17/G8965-10A_1.jpg

Picture4 Text :

Immunoprecipitation: G8956-10A analysis in HeLa cell lysate.