Green Fluorescent Protein (GFP) | US Biological G8965-10 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP)

catalog :

G8965-10

quantity :

50 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

G8965-10

Product wo Prefix :

Green Fluorescent Protein (GFP)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

50 ug

Isotype :

IgG

Desc1 :

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light. Applications: Suitable for use in Immunocytochemistry, Western Blot and ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher. Note: While this antibody has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, use highly-purified secondary antibodies in indirect detection systems. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Highly purified native GFP from Aequorea victoria

Specificity :

Reactive with GFP from both native and recombinant sources. Minimal crossreactivity with E. coli.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in 10mM Tris, pH 7.2, 0.01% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~1mg/ml Form: Supplied as a liquid in 10mM Tris, pH 7.2, 0.01% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: Highly purified native GFP from Aequorea victoria Specificity: Reactive with GFP from both native and recombinant sources. Minimal crossreactivity with E. coli. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IC WB

Storage Temperature :

-20°C

Reference :

1. Haas, Kurt, et al. (2006). Proc Natl Acad Sci USA. 103:12127-31. 2. Knott, Graham W, et al. (2006). Nat Neurosci. 9:1117-24. 3. Steele, Andrew D, et al. (2006). Proc Natl Acad Sci USA. 103:3416-21. 4. Ashby, M. C., et al. (2004). J. Neurosci. 24:5172-5176. 5. Belluzzi O., Benedusi M., Ackman J., & LoTurco J. J. (2003). J. Neurosci. 23[32]:10411-10418.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/32/G8965-10_1.jpg