Green Fluorescent Protein (GFP) | US Biological G8965-07 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP)

catalog :

G8965-07

quantity :

500 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1001

product information

Catalog Number :

G8965-07

Product wo Prefix :

Green Fluorescent Protein (GFP)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

500 ug

Clone # USB :

1001

Isotype :

IgG1,k

Desc1 :

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:80,000-1:500,000. Sandwich or capture for detection of GFP recombinant proteins and enhanced forms of GFP. Western Blot: 1:3000-1:30,000 Immunohistochemistry:1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyﬁsh Aequorea victoria.

Specificity :

Recognized wild type and recombinant forms of GFP. No reaction is seen against RFP. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti mouse serum.

Purity :

Purified by Protein A affinity chrmoatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 1001 Host: mouse Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide Purity: Purified by Protein A affinity chrmoatography. Immunogen: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyﬁsh Aequorea victoria. Specificity: Recognized wild type and recombinant forms of GFP. No reaction is seen against RFP. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti mouse serum. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C