Green Fluorescent Protein (GFP) | US Biological G8965-06 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP)

catalog :

G8965-06

quantity :

200 ug

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

product information

Catalog Number :

G8965-06

Product wo Prefix :

Green Fluorescent Protein (GFP)

Host :

chicken

Product Type :

Pab

Category :

Antibodies

Size1 :

200 ug

Isotype :

IgY

Desc1 :

Green fluorescent protein (GFP) is a 29kD fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488nm), GFP strongly fluoresces (Emission max: 507nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detects Green Fluorescent Protein from 50ug of rat brain lysate containing 25ng of purified Green Fluorescent Protein. Also detected a GFP-fusion protein from a transfected E. coli cell lysate. Rat brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8965-06 (1ug/ml). Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch Positive Control: rat brain lysate, cells expressing Green Fluorescent Protein Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

His-tagged green fluorescent protein of Aequorea victoria.

Specificity :

Recognizes Green Fluorescent Protein (GFP), Mr 30kD, and GFP-fusion proteins. Species Crossreactivity: Vertebrates

Purity :

Purified by ammonium sulfate precipitation and PEG.

Form :

Supplied as a liquid in 100mM Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Isotype: IgY Host: chicken Concentration: ~1mg/ml Form: Supplied as a liquid in 100mM Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol. Purity: Purified by ammonium sulfate precipitation and PEG. Immunogen: His-tagged green fluorescent protein of Aequorea victoria. Specificity: Recognizes Green Fluorescent Protein (GFP), Mr 30kD, and GFP-fusion proteins. Species Crossreactivity: Vertebrates Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IC WB

Storage Temperature :

4°C

Reference :

Gerdes, H. H. and Kaether, C., FEBS Letters 389: 44–47 (1996) Kain, S.R., et al., BioTechniques 19: 650–655 (1995) Davis, I. et al. Developmental Biology 170: 726-729 (1995)

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/23/G8965-06_1.jpg