Green Fluorescent Protein (GFP) | US Biological G8965-02B product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP)

catalog :

G8965-02B

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

G8965-02B

Product wo Prefix :

Green Fluorescent Protein (GFP)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Green fluorescent protein (GFP), a 27kD protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelenth of 509nm) when excited by blue light (excitation peak at a wavelenth of 395nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP is used to measure single cell metastasis and successful proliferation of stem cells. In these ways, GFP technology has contributed to a greater understanding of cellular biology and biochemistry. Applications: Suitable for use in Immunocytochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: ELISA: 1:2000-1:10,000 Western Blot: 1:1000-1:5000. Detects a band of ~28kD. using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Highly-purified native GFP from Aequorea victoria.

Specificity :

Recognizes GFP and GFP fusion proteins from both native and recombinant sources. This anti-GFP recognizes all variants of A. victoria GFP induced in transformed organisms, including all vertebrate species tested.

Purity :

Purified by immunoaffinity chromatography. >99% by SDS-PAGE & HPLC

Form :

Supplied as a liquid in TBS, pH 7.2, , 0.01% sodium azide.

Concentration :

~4mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: ~4mg/ml Form: Supplied as a liquid in TBS, pH 7.2, , 0.01% sodium azide. Purity: Purified by immunoaffinity chromatography. >99% by SDS-PAGE & HPLC Immunogen: Highly-purified native GFP from Aequorea victoria. Specificity: Recognizes GFP and GFP fusion proteins from both native and recombinant sources. This anti-GFP recognizes all variants of A. victoria GFP induced in transformed organisms, including all vertebrate species tested. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

Understanding, improving and using green fluorescent proteins: A.B. Cubitt, et al.; TIBS 20, 448 (1995) Chalfie, M., Y. Tu, G. Euskirchen, W.W. Ward and D.C. Prasher. 1994. Green-fluorescent protein as a marker for gene expression. Science 263:802-805.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/G8965-02B_1.jpg