antibody

Green Fluorescent Protein (GFP)

G8965-01E

100 ug

706 USD

polyclonal

domestic goat

nonconjugated

Green Fluorescent Protein (GFP)

goat

Pab

Antibodies

100 ug

706 USD

IgG

Green Fluorescent Protein (GFP) is a 27kD protein derived from the jellyfish Aquorea victoria, which emits green light (emission peak at a wavelenth of 509nm) when excited by blue light (excitation peak at a wavelenth of 395nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence/Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:40,000 Western Blot: 1:400-1:2000 Immunofluorescence (IC): 1:10-1:500 Immunoprecipitation: 1:10-1:500 Immunohistochemistry (Frozen): 1:10-1:500 Immunohistochemistry (Paraffin): 1:10-1:500 Flow Cytometry: 1:10-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dr Hu Mo

Green Fluorescent Protein

Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Recognizes Green Fluorescent Protein. When used in ELISA, direct binding of antigen recognizes wild type, recombinant and enhanced forms of GFP. Assay by Immunoelectrophoresis resulted in single precipitin arc against anti-goat serum and purified Green Fluorescent Protein (Aequorea victoria). Species Crossreactivity: human, mouse and Drosophila.

Purified by immunoaffinity chromatography

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

~1mg/ml

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. Purity: Purified by immunoaffinity chromatography Immunogen: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. Specificity: Recognizes Green Fluorescent Protein. When used in ELISA, direct binding of antigen recognizes wild type, recombinant and enhanced forms of GFP. Assay by Immunoelectrophoresis resulted in single precipitin arc against anti-goat serum and purified Green Fluorescent Protein (Aequorea victoria). Species Crossreactivity: human, mouse and Drosophila. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IC IF IHC IP WB

-20°C

1. Shafikhani, S.H., and Engel, J., PNAS 103: 15,605-15,610 (2006). 2. Yano, M., et al., J. Exp. Med. 205(12): 2827-2838 (2008). 3. Yee, M., et al., Am. J. Physiol. Lung Cell Mol. Physiol. 291: L1101-1111 (2006).

https://usbio-images.r.worldssl.net/prodimages/4/G8965-01E_1.jpg

Mouse brain using G8965-01E (coronal view, 20X magnification).

https://usbio-images.r.worldssl.net/prodimages/4/G8965-01E_5.jpg

Immunofluorescence analysis of GFP in mouse brain