Green Fluorescent Protein (GFP) | US Biological G8965-01D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP)

catalog :

G8965-01D

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8H198

product information

Catalog Number :

G8965-01D

Product wo Prefix :

Green Fluorescent Protein (GFP)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

8H198

Isotype :

IgG2a

Desc1 :

The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27kD. GFP is in the shape of a cylinder, comprising 11 strands of beta-sheet outside, a single alpha helical strand containing the chromophore running through the center, and short helical segments on the ends. GFP transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. GFP has been widely used as a tracer in various diciplines of biology research, such as fluorescence microscopy, reporter gene expression, and in vivo imaging. Laboratory mutation of GFP generated the widely used analog GFPuv, which has brighter fluorescence and more desirable expression properties. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml Detection limit for rGFPuv ~ 1ng/well. Western Blot: 1-2ug/ml Detection limit for rGFPuv ~ 5ng/lane under non-reducing and reducing conditions. Immunoprecipitation: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Recombinant GFP, aa 2-238 (E. coli).

Specificity :

Recognizes recombinant GFPuv.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.

Concentration :

As reported

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 8H198 Host: mouse Concentration: As reported Form: Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant GFP, aa 2-238 (E. coli). Specificity: Recognizes recombinant GFPuv. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C