antibody

Green Fluorescent Protein (GFP)

G8965-01A

100 ul

polyclonal

domestic rabbit

nonconjugated

Green Fluorescent Protein (GFP)

rabbit

Pab

Antibodies

100 ul

IgG

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their size, they are unlikely to affect the tagged protein’s biochemical properties. The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395nm and has an emission spectrum that peaks at 509nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations (1). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: 1:1600. Useful for paraffin-embedded transfected cells only. Antigen retrieval by heating in citrate /TBST buffer is required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide corresponding to GFP.

Recognizes GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27kD to the molecular weight of the fusion protein.

Purified b Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified b Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to GFP. Specificity: Recognizes GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27kD to the molecular weight of the fusion protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IHC WB

-20°C

1. Miyawaki, A. et al. (2003) Curr. Opin. Chem. Biol. 7, 557–562.

https://usbio-images.r.worldssl.net/prodimages/18/G8965-01A_1.jpg