Green Fluorescent Protein (GFP) | US Biological G8965-01 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Green Fluorescent Protein (GFP)

catalog :

G8965-01

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GF200

product information

Catalog Number :

G8965-01

Product wo Prefix :

Green Fluorescent Protein (GFP)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

GF200

Isotype :

IgG1,k

Desc1 :

GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent and suitable for a variety of applications. Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 ELISA: 1:2000-1:20,000 Optimal dilutions to be determined by the researcher. Hybridoma: Mouse GF200 Source: Ascites Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Purified recombinant His-GFP full length fusion protein (Aequorea victoria).

Specificity :

Reacts with Green Fluorescent Protein (GFP) and crossreacts with Blue Fluorescent Protein (BFP).

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.02% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: GF200 Host: mouse Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 0.02% sodium azide. Purity: Purified by Protein G affinity chromatography. Immunogen: Purified recombinant His-GFP full length fusion protein (Aequorea victoria). Specificity: Reacts with Green Fluorescent Protein (GFP) and crossreacts with Blue Fluorescent Protein (BFP). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Prasher, D.C., et al. (1992) Gene 111:229-233 (1982). 2. Chalfie, M., et al. (1994) Science 263:802-805 (1994). 3. Youvan, D.C. (1995) Science 268:264 (1995). 4. Ilic, D., et al; J. Cell Biol. 143(2):547-560 (1998).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/33/G8965-01_1.jpg