Glycogen Synthase Kinase 3 alpha, beta, phosphorylated (Ser21/9) (GSK3a,b) | US Biological G8170-43B product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Glycogen Synthase Kinase 3 alpha, beta, phosphorylated (Ser21/9) (GSK3a,b)

catalog :

G8170-43B

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

G8170-43B

Product wo Prefix :

Glycogen Synthase Kinase 3 alpha, beta, phosphorylated (Ser21/9) (GSK3a,b)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Glycogen synthase kinase 3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3alpha and Ser9 of GSK-3beta. GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50. Use citrate as unmasking buffer. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to the sequence of human GSK-3 alpha.

Specificity :

Recognizes endogenous levels of human GSK-3 only when phosphorylated at Ser21 of GSK-3 alpha or Ser9 of GSK-3 beta. MW: ~46, 51kD. Species Crossreactivity: mouse, rat, monkey and zebrafish

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to the sequence of human GSK-3 alpha. Specificity: Recognizes endogenous levels of human GSK-3 only when phosphorylated at Ser21 of GSK-3 alpha or Ser9 of GSK-3 beta. MW: ~46, 51kD. Species Crossreactivity: mouse, rat, monkey and zebrafish Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Welsh, G. I. et al. (1996) Trends Cell. Biol. 6, 274–279. 2. Srivastava, A.K. and Pandey, S.K. (1999) Mol. Cell. Biochem. 182, 135–141. 3. Cross, D. A. et al. (1995) Nature 378, 785–789. 4. Nusse, R. (1997) Cell 89, 321–323. 5. Diehl, J. A. et al. (1998) Genes Dev. 12, 3499–3511.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/26/G8170-43B_1.jpg