Glycogen Synthase Kinase 3 alpha, phosphorylated (Ser21) (GSK3a) | US Biological G8170-42C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Glycogen Synthase Kinase 3 alpha, phosphorylated (Ser21) (GSK3a)

catalog :

G8170-42C

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

3i16(46H12)

reactivity :

human

product information

Catalog Number :

G8170-42C

Product wo Prefix :

Glycogen Synthase Kinase 3 alpha, phosphorylated (Ser21) (GSK3a)

Host :

mouse

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

3i16(46H12)

Isotype :

IgG2b

Desc1 :

Glycogen synthase kinase 3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3alpha and Ser9 of GSK-3beta (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt Ze

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3alpha.

Specificity :

Detects endogenous levels of GSK-3 alpha only when phosphorylated at Ser21. Species Crossreactivity: Human, mouse, rat, monkey and zebrafish.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 3i16(46H12) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, less than 0.02% sodium azide, 50% glycerol. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3alpha. Specificity: Detects endogenous levels of GSK-3 alpha only when phosphorylated at Ser21. Species Crossreactivity: Human, mouse, rat, monkey and zebrafish. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Welsh, G.I. et al. (1996) Trends Cell Biol. 6, 274–279. (2) Srivastava, A.K. and Pandey, S.K. (1999) Mol. Cell. Biochem. 182, 135–141. (3) Cross, D.A. et al. (1995) Nature 378, 785–789. (4) Nusse, R. (1997) Cell 89, 321–323. (5) Diehl, J.A. et al. (1998) Genes Dev. 12, 3499–3511.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/27/G8170-42C_1.jpg